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KMID : 0903519900330030239
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1990 Volume.33 No. 3 p.239 ~ p.246
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Pigment production in Monascus anka
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Abstract
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Monascus anka strains with higher pigment production were developed using UV mutation and natural selection. To obtain organic solvent soluble pigments from Manascus anka, the following culture conditions were compared; standing and shaking culture with Nishikawa´s medium, and shaking culture with Lin´s medium. Shaking culture in Lin´s medium exhibited decrease in solvent.-soluble intracellular pigments after initial increase. The decrease was accompanied by the increase in water-soluble extracellular pigments. Manascus anka preferred sucrose and ethanol among 7 carbon sources tested. Treatment of sterol biosynthesis inhibitors, (±)-miconazole and chlorocholine chloride(CCC), directed carbon pool to the biosynthetic pathway leading to the pigments with CCC´s more pronounced effect. Two dimensional TLC revealed at least 7 yellow pigments suggesting existence of hereto unreported pigment. One of the most abundant yellow pigments was isolated and found to be ankaflavin by NMR and MS analysis.
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